production and application of monoclonal antibodies pdf

Production and application of monoclonal antibodies pdf

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Producing Polyclonal Antibodies

Polyclonal and Monoclonal Antibody Production

1st Edition

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The currently available methods for the production of human monoclonal antibodies Mabs are: mouse-human hybrids, human-human hybrids, EBV transformation, and fusion of EBV-transformed specific B-lymphocytes with a malignant cell line. Each method has its advantages and drawbacks, but they are all inferior to an optimal functioning mouse hybridoma system. A significant obstacle is the lack of a reproducible method to obtain high amounts of antigen-specific B-lymphocytes.

Producing Polyclonal Antibodies

The currently available methods for the production of human monoclonal antibodies Mabs are: mouse-human hybrids, human-human hybrids, EBV transformation, and fusion of EBV-transformed specific B-lymphocytes with a malignant cell line. Each method has its advantages and drawbacks, but they are all inferior to an optimal functioning mouse hybridoma system.

A significant obstacle is the lack of a reproducible method to obtain high amounts of antigen-specific B-lymphocytes. However, it seems that newly developed in vitro antigen-priming methods may be a significant aid to the solution of this problem. Human Mabs have already been obtained against some types of human cancer, and the reactivity pattern has provided some biological information that seems not to be obtainable with non-human Mabs. It furthermore seems that the technology for production of human Mabs now has progressed to the stage where it permits a realistic hope that they soon can be applied in the diagnosis and treatment of human cancer.

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Polyclonal and Monoclonal Antibody Production

Monoclonal Antibodies are cells derived by cell division from a single ancestral cell. Monoclonals are a class of antibodies with identical offspring of a hybridoma and are very specific for a particular location in the body derived from a single clone and can be grown indefinitely. Monoclonal Antibodies recognize and bind to antigens in order to discriminate between specific epitopes which provides protection against disease organisms. Monoclonal antibodies target various proteins that influence cell activity such as receptors or other proteins present on the surface of normal and cancer cells. The specificity of Monoclonal Antibodies allows its binding to cancerous cells by coupling a cytotoxic agent such as a strong radioactive which then seek outs to destroy the cancer cells while not harming the healthy ones.

1st Edition

General properties and applications of monoclonal antibodies. Assay techniques. Selection of animals and cell lines. Cell culture requirements for hybridomas.

This article throws light upon the four types of applications of monoclonal antibodies. Monoclonal antibodies have revolutionized the laboratory diagnosis of various diseases. For this purpose, MAbs may be employed as diagnostic reagents for biochemical analysis or as tools for diagnostic imaging of diseases.

Monoclonal Antibodies

Monoclonal antibodies Mabs Antibodies are glycoprotein synthesized in blood against specific antigens hust to combat and give immunity.

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DOI : Since the advent of hybridoma technology 35 years ago, research on monoclonal antibodies has developed enormously. Monoclonal antibodies of mouse origin were the first to be produced and continue to be the most popular affinity reagents for investigating the proteome of all organisms. For their adaptability to a variety of biological assays monoclonal antibodies are key tools for basic research as well as for diagnosis and therapy of human diseases. Recently, the expanding demand of high-quality antibodies with better specificities has resulted in a significant improvement in traditional hybridoma production methods.

Basic Immunology "Function and disorder of the immune system". Williams and Wilkins 2 nd Ed:. Monoclonal Antibodies: A Review.

Marlies Leenaars, Ph.

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